James Mbora Wainaina

Molecular techniques

 

• Bacteria and fungal culturing

• Cell culturing (HEK293) and isolation

• Western Blot assay’s

• Conventional PCR, RT-qPCR, and RT-PCR

• Genotyping using microsatellite markers (SSR)

• Next-generation sequencing (DNA-Seq, RNA-Seq)

• Enzyme-linked immunosorbent assay (ELISA)

 

Computational biology techniques

 

• Assembly of next-generation data (2nd and 3rd generation) sequencing platforms

• Bayesian and Maximum likelihood phylogenetic analysis

• Evolutionary and selective pressure analysis

• Virus recombination analysis

• Discrete phylogeography analysis of viruses

• Population genetic and gene flow estimations

 

Languages and scripting

 

• Perl 


• Bash 


• Unix and Linux 


• R